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LiveReport™ NFκB Assay Kit

LiveReport™ NFκB Assay Kit

The LiveReport™ NFkB assay kit is a non-destructive solution for tracking Nuclear Factor-kB (NFkB) modulation in real-time.

Using the LiveReport™ NFkB kit you can assay host cell NFkB modulation continuously or repeatedly without perturbing the cells under study.

Cells treated with the LiveReport™ NFkB assay kit continuously and automatically adjust their bioluminescent intensity to reflect real-time NFkB modulation dynamics without any external stimulation required, providing a live stream of response data.

  • 10 μL Sample Try for free*

    Sufficient to perform 10 reactions in the 96-well format or 40 reactions in the 384-well format.

  • 100 μL Kit $225

    Sufficient to perform 100 reactions in the 96-well format or 400 reactions in the 384-well format.

  • 1,000 μL Kit $2,000

    Sufficient to perform 1,000 reactions in the 96-well format or 4,000 reactions in the 384-well format.

*Includes free shipping within the United States.
$50 value. New customers only. Limit one (1) per customer. While supplies last.

Easy To Use

The LiveReport™ NFκB assay kit is a non-destructive solution for tracking Nuclear Factor-κB (NFκB) modulation in real-time.

Using the LiveReport™ NFκB kit you can assay host cell NFκB modulation continuously or repeatedly without perturbing the cells under study.

Cells treated with LiveReport™ NFκB assay mix express both luciferase and luciferin synthesis enzymes and continuously and automatically adjust their bioluminescent intensity to reflect real-time NFκB modulation dynamics without any external stimulation required.

The LiveReport™ NFκB assay mix can be transfected into cells using any standard method. After reagent uptake and expression, NFκB modulation can be assayed repeatedly or continuously for up to one week or more, providing a live stream of response data.

Continuous, Automatic Performance

The LiveReport™ NFκB assay mix contains:

    1. DNA for luciferase enzyme synthesis

    2. DNA for luciferin generation enzyme synthesis

    3. All external co-factors required for continuous bioluminescence

The supplied mixture is transfected into the cell to introduce the DNA for transcription/translation. Simultaneously, the co-factors are delivered into the medium for cellular uptake. Immediately after translation of the luciferin synthesis portion of the system, it will begin to scavenge cytosolically available components and convert them into luciferin for the bioluminescent reaction. The luciferin synthesis system has been specifically engineered so that the scavenging process does not impact cellular health or physiology. This ensures high data integrity and prevents luciferin synthesis from interfering with native metabolic processes.

The co-expressed luciferase enzyme requires luciferin (supplied by the luciferin synthesis complex), oxygen (available in abundance under routine culture conditions), and reducing power to produce light. Activation of the bioluminescent system is controlled by multiple NFκB response elements placed strategically upstream of a minimal promoter. When the NFκB response factors are activated, light production is stimulated. The level of stimulation correlates with bioluminescent signal intensity, making it easy to quickly determine the timing, magnitude, and duration of stimulation.