Autobioluminescent cells can often be used in place of substrate-dependent cell lines to simplify assay design, reduce costs, and increase the amount of data collected in your assays. The autonomous nature of the autobioluminescent signal allows cells to be assayed repeatedly or continuously without destruction, reducing the number of cells that must be prepared for each experiment and reducing sample to sample variability. Because the autobioluminescent signal is completely self-modulated and does not require chemical or photonic stimulation prior to emission, autobioluminescent cells are also more amenable to automated or high throughput assay designs. From a quality control standpoint, the use of autobioluminescent cells removes concerns relating to substrate quality, uptake rates, or application efficiency, and completely removes any chance for unintended substrate/treatment interactions. In general, if a substrate-dependent cell line is used, an autobioluminescent cell line can be substituted to perform the same assay with less cost and less investigator effort, all while providing you with increased data acquisition through continuous monitoring.