It’s time your assays started working harder for you. Free yourself from the need for fluorescent excitation or bioluminescent luciferin treatment. Autobioluminescence scavenges small amounts of intracellular chemicals to make and recycle luciferin so cells can autonomously and repeatedly turn on or off light production, or continuously modulate light intensity to report real-time viability/metabolic activity.
How It Works
In a traditional bioluminescent reaction, the cell produces the luciferase and the metabolic power to support the reaction. The user must lyse the cell to release these components and apply a chemically synthesized luciferin. This results in the death of the cells under study and produces only a single time point of information.
490 BioTech’s autobioluminescent technology bypasses the need for cellular destruction and luciferin treatment by enabling the cell to assemble and recycle the luciferin component from cytosolically available components. This allows cells to produce light continuously, or to autonomously turn on or off light production without any external intervention. Data is provided continuously and the cells remain unperturbed so they can be assayed repeatedly or used for further experimentation.
Autobioluminescence has been optimized so that only cytosolic components are used, and only at a level that does not impact native physiology. This allows autobioluminescent cells to remain physiologically identical to wild-type, non-luminescent cells so you can be confident the data you get will be reliable and actionable.
Pre-made autobioluminescent cell lines have been engineered to continuously modulate their bioluminescent intensity to reflect real-time changes in metabolic activity.
From the moment they’re thawed, these cell lines produce a bioluminescent signal. The autobioluminescent phenotype is passed down to all daughter cells, allowing populations to be monitored indefinitely. This makes them ideal for in vivo xenograft studies because health and viability dynamics can be monitored repeatedly without needle stick or fluorescent background.
Xenobiotic compounds and naturally occurring chemicals can lead to disruption of the endocrine system in vertebrates. Autobioluminescent yeast (Saccharomyces cerevisiae) detect estrogenic and/or androgenic activity in environmental samples at greater speeds and sensitivities than human cells, while remaining robust enough for industrial applications.
Available as individual strains or money-saving kits, these yeast strains give our industrial customers a fast, easy, robust, and inexpensive way to track toxicity and endrocrine disruption without the need for BSL 2 level facilities.