LiveReport™ TCF-LEF Assay Kit
The LiveReport™ TCF-LEF assay kit is a non-destructive solution for tracking TCF-LEF response element modulation of Wnt/B-catenin signaling pathways in real-time.
Using the LiveReport™ TCF-LEF kit you can assay host cell TCF-LEF modulation continuously or repeatedly without perturbing the cells under study.
Cells treated with the LiveReport™ TCF-LEF assay kit continuously and automatically adjust their bioluminescent intensity to reflect real-time TCF-LEF modulation dynamics without any external stimulation required, providing a live stream of response data.
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10 μL Sample Try for free*
Sufficient to perform 10 reactions in the 96-well format or 40 reactions in the 384-well format.
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100 μL Kit $225
Sufficient to perform 100 reactions in the 96-well format or 400 reactions in the 384-well format.
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1,000 μL Kit $2,000
Sufficient to perform 1,000 reactions in the 96-well format or 4,000 reactions in the 384-well format.
*Includes free shipping within the United States.
$50 value. New customers only. Limit one (1) per customer. While supplies last.
Easy To Use
The LiveReport™ TCF-LEF assay kit is a non-destructive solution for tracking TCF-LEF modulation of Wnt/B-catenin signaling pathways in real-time.
Using the LiveReport™ TCF-LEF kit you can assay host cell TCF-LEF modulation continuously or repeatedly without perturbing the cells under study.
Cells treated with LiveReport™ TCF-LEF assay mix express both luciferase and luciferin synthesis enzymes and continuously and automatically adjust their bioluminescent intensity to reflect real-time TCF-LEF modulation dynamics without any external stimulation required.
The LiveReport™ TCF-LEF assay mix can be transfected into cells using any standard method. After reagent uptake and expression, TCF-LEF modulation can be assayed repeatedly or continuously for up to one week or more, providing a live stream of response data.
Continuous, Automatic Performance
The LiveReport™ TCF-LEF assay mix contains:
DNA for luciferase enzyme synthesis
DNA for luciferin generation enzyme synthesis
All external co-factors required for continuous bioluminescence
The supplied mixture is transfected into the cell to introduce the DNA for transcription/translation. Simultaneously, the co-factors are delivered into the medium for cellular uptake. Immediately after translation of the luciferin synthesis portion of the system, it will begin to scavenge cytosolically available components and convert them into luciferin for the bioluminescent reaction. The luciferin synthesis system has been specifically engineered so that the scavenging process does not impact cellular health or physiology. This ensures high data integrity and prevents luciferin synthesis from interfering with native metabolic processes.
The co-expressed luciferase enzyme requires luciferin (supplied by the luciferin synthesis complex), oxygen (available in abundance under routine culture conditions), and reducing power to produce light. Activation of the bioluminescent system is controlled by multiple TCF-LEF response elements placed strategically upstream of a minimal promoter. When the TCF-LEF response elements are activated, light production is stimulated. The level of stimulation correlates with bioluminescent signal intensity, making it easy to quickly determine the timing, magnitude, and duration of stimulation.
