LiveLight™ Toxicity Assay Kit
The LiveLight™ Toxicity Assay Kit is a non-destructive solution for tracking cellular health in real-time.
Using the LiveLight™ Toxicity Assay Kit you can assay viability continuously or repeatedly without perturbing the cells under study.
Cells treated with the LiveLight™ Toxicity Assay Kit continuously and automatically adjust their bioluminescent intensity to reflect real-time metabolic activity dynamics without any external stimulation required. Healthy cells glow brightly, unhealthy cells become dim, and dead cells produce no light.
Kinetic luminescence monitoring of HEK293 cells challenged with ellipticine reveals the timing of drug metabolism and the resulting effects on metabolic activity. At low doses (500 nM), ellipticine increases metabolic activity by uncoupling oxidative phosphorylation. At high doses (5 μM), it induces cytotoxicity by depleting ATP availability. As ellipticine is metabolized, the bioavailable dose transitions from high to low, resulting in dynamic metabolic effects (n = 3).
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10 μL Sample Try for free*
Sufficient to perform 10 reactions in the 96-well format or 40 reactions in the 384-well format.
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100 μL Kit $225
Sufficient to perform 100 reactions in the 96-well format or 400 reactions in the 384-well format.
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1,000 μL Kit $2,000
Sufficient to perform 1,000 reactions in the 96-well format or 4,000 reactions in the 384-well format.
*Includes free shipping within the United States.
$50 value. New customers only. Limit one (1) per customer. While supplies last.
Easy To Use
The LiveLight™ Toxicity Assay Kit is a non-destructive solution for tracking cellular health in real-time.
Using the LiveLight™ Toxicity Assay Kit you can assay viability continuously or repeatedly without perturbing the cells under study.
Cells treated with LiveLight™ Toxicity Assay mix express both luciferase and luciferin synthesis enzymes and continuously and automatically adjust their bioluminescent intensity to reflect real-time metabolic activity dynamics without any external stimulation required.
The LiveLight™ Toxicity Assay Mix can be transfected into cells using any standard method. After reagent uptake and expression, metabolic activity/viability can be assayed repeatedly or continuously for up to one week or more, providing a live stream of cellular health data.
Continuous, Automatic Performance
The LiveLight™ Toxicity Assay Mix contains:
DNA for luciferase enzyme synthesis
DNA for luciferin generation enzyme synthesis
All external co-factors required for continuous bioluminescence
The supplied mixture is transfected into the cell to introduce the DNA for transcription/translation. Simultaneously, the co-factors are delivered into the medium for cellular uptake. Immediately after translation of the luciferin synthesis portion of the system, it will begin to scavenge cytosolically available components and convert them into luciferin for the bioluminescent reaction. The luciferin synthesis system has been specifically engineered so that the scavenging process does not impact cellular health or physiology. This ensures high data integrity and prevents luciferin synthesis from interfering with native metabolic processes.
The co-expressed luciferase enzyme requires luciferin (supplied by the luciferin synthesis complex), oxygen (available in abundance under routine culture conditions), and reducing power to produce light. The availability of the reducing power component, which parallels host metabolic state, acts as the limiting reagent and allows for continuous and immediate modulation of bioluminescent intensity.
The result is a continuously adjusted bioluminescent signal from each cell in the population that reflects its real-time level of health. The more metabolically active the cell, the brighter the signal. Dead cells, which have no more metabolic activity, are incapable of producing light.
