LiveLight™ Toxicity Assay Kit
The LiveLight™ Toxicity Assay Kit is a non-destructive solution for tracking cellular health in real-time.
Using the LiveLight™ Toxicity Assay Kit you can assay viability continuously or repeatedly without perturbing the cells under study.
Cells treated with the LiveLight™ Toxicity Assay Kit continuously and automatically adjust their bioluminescent intensity to reflect real-time metabolic activity dynamics without any external stimulation required. Healthy cells glow brightly, unhealthy cells become dim, and dead cells produce no light.
Kinetic luminescence monitoring of HEK293 cells challenged with ellipticine reveals the timing of drug metabolism and the resulting effects on metabolic activity. At low doses (500 nM), ellipticine increases metabolic activity by uncoupling oxidative phosphorylation. At high doses (5 μM), it induces cytotoxicity by depleting ATP availability. As ellipticine is metabolized, the bioavailable dose transitions from high to low, resulting in dynamic metabolic effects (n = 3).
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10 μL Sample Try for free*
Sufficient to perform 10 reactions in the 96-well format or 40 reactions in the 384-well format.
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100 μL Kit $225
Sufficient to perform 100 reactions in the 96-well format or 400 reactions in the 384-well format.
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1,000 μL Kit $2,000
Sufficient to perform 1,000 reactions in the 96-well format or 4,000 reactions in the 384-well format.
*Includes free shipping within the United States.
$50 value. New customers only. Limit one (1) per customer. While supplies last.
Easy To Use
The LiveLight™ Toxicity Assay Kit is a non-destructive solution for tracking cellular health in real-time.
Using the LiveLight™ Toxicity Assay Kit you can assay viability continuously or repeatedly without perturbing the cells under study.
Cells treated with LiveLight™ Toxicity Assay mix express both luciferase and luciferin synthesis enzymes and continuously and automatically adjust their bioluminescent intensity to reflect real-time metabolic activity dynamics without any external stimulation required.
The LiveLight™ Toxicity Assay Mix can be transfected into cells using any standard method. After reagent uptake and expression, metabolic activity/viability can be assayed repeatedly or continuously for up to one week or more, providing a live stream of cellular health data.
Continuous, Automatic Performance
The LiveLight™ Toxicity Assay Mix contains:
DNA for luciferase enzyme synthesis
DNA for luciferin generation enzyme synthesis
All external co-factors required for continuous bioluminescence